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Nega-Gosalyn is the counterpart of Gosalyn Mallard, living in the Negaverse. Unlike Gosalyn, she wears a dress and appears to be very girly, sweet, obedient, sentimental, and compliant. She is innocent and does not seem to have the spirit to fight.

Her adoptive father is Negaduck but he doesn't seem to take care of her at all. Nega-Launchpad, however, gives her a lot of presents, which implies that Negaduck certainly orders him to do so and Nega-Gosalyn would then be a spoiled child, even if she doesn't act like one at all.

When Darkwing Duck arrives in the Negaverse, she first thinks it is Negaduck, her father. When she realizes that this is Darkwing Duck, she is very happy about it. Darkwing realizes that there is no "Darkwing Duck" to take care of Gosalyn in this dimension and he is then determined to save her.

Later, when Negaduck is defeated, the Friendly Four became her guardians.

She was voiced by the late Christine Cavanaugh.